Hands on Workshop on Human Cell Culture Pune
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Hands on Workshop on Human Cell Culture is under conferences in Pune, workshops in Pune, conferences and workshops and conferences in india 2018.
1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques, Molecular Biology & MTT Assay, Pune, India. For dates and registration please click the following website: http://icsccb.org/workshops/cellcultureworkshop/
Following techniques will be covered using human embryonic kidney cell line and human blood cancer cell lines:
1. Animal cell culture techniques, passaging and maintenance of cell lines
2. Media preparation
3. Cryopreservation (freezing and thawing of cell lines)
4. Genomic DNA isolation from cancer cell lines,
5. DNA estimation
6. DNA agarose gel electrophoresis
7. Total RNA extraction
8. cDNA synthesis from RNA (Reverse Transcription),
9. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
10. Agarose gel electrophoresis of PCR product
11. Total protein extraction
12. Protein estimation
13. SDS-PAGE electrophoresis of extracted proteins
14. Coomassie staining of protein gel
15. MTT Assay for cell proliferation/cytotoxicity
This is a hands-on workshop intended for beginners to teach and train them in animal cell culture and molecular biology techniques using the human/cancer cell lines for various experiments. It will be supported by some lectures on basic cell culture techniques, media preparation, gene/protein expression & interaction networks in hematopoietic stem cells (HSCs) and cancer cells.
Type of Event: Conferences in Pune.
Category of Event: Workshops in Pune.
Hands on Workshop on Human Cell Culture is categorized under Pune Conferences Near Me, Pune Workshops Around Me, conferences and workshops in Pune 2018, Workshops and Conferences today, and India Conferences Near Me.
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